Conversion of Reduced Nicotinamide-Adenine Dinucleotide Dehydrogenase into Reduced Nicotinamide-Adenine Dinucleotide-Ubiquinone Reductase
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چکیده
منابع مشابه
Formation of reduced nicotinamide adenine dinucleotide peroxide.
Incubation of NADH at neutral and slightly alkaline pH leads to the gradual absorption of 1 mol of H+. This uptake of acid requires oxygen and mainly yields anomerized NAD+ (NAD+), with only minimal formation od acid-modified NADH. The overall stoichiometry of the reaction is: NADH + H+ + 1/2O2 leads to H2O + NAD+, with NADH peroxide (HO2-NADH+) serving as the intermediate that anomerizes and b...
متن کاملInhibition of rat liver nicotinamide adenine dinucleotide kinase by reduced nicotinamide adenine dinucleotide phosphate.
Rat liver NAD kinase (ATP : NAD 2’-phosphotransferase, EC 2.7.1.23) was purified about 70-fold. The MichaelisMenten constants (Km) for NAD and ATP were 8 x 10e4 M and 2 x low3 M, respectively. NAD kinase activity was markedly inhibited by NADH and also NADPH. The Ki of NADH was approximately 1 X 10q4 M, and that of NADPH was approximately 5 X 10M5 M. Both inhibitions were competitive with NAD, ...
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A mitochondrial defect was investigated in an infant with fatal congenital lactic acidosis (3-14 mM), high lactate-to-pyruvate ratio, hypotonia, and cardiomyopathy. His sister had died with a similar disorder. Resting oxygen consumption was 150% of controls. Pathological findings included increased numbers of skeletal muscle mitochondria (many with proliferated, concentric cristae), cardiomegal...
متن کاملReduced nicotinamide-adenine dinucleotide-nitrite reductase from Azotobacter chroococcum.
1. The assimilatory nitrite reductase of the N(2)-fixing bacterium Azotobacter chroococcum was prepared in a soluble form from cells grown aerobically with nitrate as the nitrogen source, and some of its properties have been studied. 2. The enzyme is a FAD-dependent metalloprotein (mol.wt. about 67000), which stoicheiometrically catalyses the direct reduction of nitrite to NH(3) with NADH as th...
متن کاملStudies on the respiratory chain-linked reduced nicotinamide adenine dinucleotide dehydrogenase. XI. Transformation of the dehydrogenase to reduced nicotinamide adenine dinucleotide-coenzyme Q reductase.
Purified preparations of the reduced nicotinamide adenine dinucleotide dehydrogenase of the respiratory chain, extracted with the aid of phospholipase at moderate temperature, do not catalyze the reduction of long chain coenzyme Q derivatives (Q6 and QIO) at significant rates and are not inhibited by amytal or rotenone. On exposure of the enzyme to conditions which have been used for the extrac...
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ژورنال
عنوان ژورنال: Biochemical Journal
سال: 1967
ISSN: 0006-2936
DOI: 10.1042/bj1040022c